Cd- and Zn-NagA ended up used to deal with the incidence of immediate interactions in the carbonyl oxygen/sulfur and the metal ion via a comparison on the kinetic constants for Nwas inactivated with the removing in the zinc certain to the Lively website plus the apo-enzyme reactivated upon incubation with 1 equivalent of Zn2+, Cd2+, Co2+, Mn2+, Ni2